Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Super enhancer-driven LINC01013 mediates hypoxia-induced mitochondrial dysfunction by HSPA9 to determine pulmonary arterial smooth muscle cell fate

doi: 10.1007/s00018-025-06071-3

Figure Lengend Snippet: Silencing of LINC01013 inhibited hypoxia-induced hPASMC proliferation and inflammation. A , B hPASMC proliferation was determined by CCK8 and EdU incorporation assays ( n = 6). EdU (red), DAPI (blue), scale bar, 50 μm. C Western blotting analysis of PCNA, cyclin A and cyclin D in hPASMCs ( n = 6). D RT‒qPCR analysis showed the mRNA levels of TNF-α and IL-6 after LINC01013 knockdown, β-actin was used as a internal reference gene ( n = 6). E Western blotting analysis of TNF-α and IL-6 in hPASMCs. ( n = 6). All values are presented as the mean ± SEM. Statistical analysis was performed with one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001. Nor, normoxia; Hyp, hypoxia; NC, negative control; si, siRNA

Article Snippet: The antibody against CEBPB (PB9171, BA0670, 1:500, Boster, Wuhan, China), H3K27ac (A7253, 1:500, ABclonal, Wuhan, China), H3K4me1 (A2355, 1:500, Wuhan, China), PCNA (A00125, 1:500, Boster, Wuhan, China), Cyclin A (PB0515, 1:500, Boster, Wuhan, China), Cyclin D (BM4272, 1:500, Boster, Wuhan, China), IL-6 (AF7236, 1:500, Beyotime, Shanghai, China), TNF-α (AF8208, 1:500, Beyotime, Shanghai, China), PKM2 (4053, 1:1000, Cell Signaling, MA, US), HK II (66974-1-Ig, 1:1000, Proteintech, IL, USA), PDH (2784, 1:1000, Cell Signaling, MA, US), HSPA9 (14887-1-AP, 1:5000, Proteintech, IL, USA), VDAC1 (10866-1-AP, 1:5000, Proteintech, IL, USA), and β-actin (TA-09, 1:1000, ZSGB‐BIO, Beijing, China) was incubated at 4 °C overnight, followed by incubation with appropriate horseradish peroxidase-conjugated secondary antibodies at room temperature for 1 h, and proteins were visualized with enhanced chemiluminescence reagents.

Techniques: Western Blot, Knockdown, Negative Control

Journal: Cancer science

Article Title: Dietary supplementation with branched-chain amino acids suppresses diethylnitrosamine-induced liver tumorigenesis in obese and diabetic C57BL/KsJ-db/db mice.

doi: 10.1111/j.1349-7006.2009.01402.x

Figure Lengend Snippet: Fig. 8. Effect of branched-chain amino acid (BCAA) supplementation on hepatic cell proliferation in diethylnitrosamine-treated C57BL ⁄ KsJ- db ⁄ db mice. (a) Immunohistochemical expression of proliferating cell nuclear antigen (PCNA) in the liver of basal diet (CRF-1)-fed, or casein- or BCAA-supplemented treated mice. (b) PCNA-labeling index in non- lesional hepatocytes was determined by counting the PCNA-positive nuclei in the hepatocytes. *P < 0.05; **P < 0.01.

Article Snippet: Immunohistochemistry of a-SMA, an indicator of HSC activation, was carried out using a primary anti-a-SMA antibody (Dako, Glostrup, Denmark). (22) Immunohistochemistry of PCNA, a G1-to-S phase marker, was carried out to estimate the cell proliferative activity of the hepatocyte using a primary anti-PCNA antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA). (23) PCNA-positive nuclei in the hepatocytes were counted and expressed as the percentage of the total number of hepatocyte nuclei.

Techniques: Immunohistochemical staining, Expressing, Labeling